分子诊断原料

MedixMDx qGreen Mix is a universal intercalating dye mix for robust, sensitive, and fast qPCR. MedixMDx qGreen Mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq polymerase and intercalating dye for real-time PCR amplification. The optimized buffer chemistry and PCR enhancers and stabilizers enable rapid and sensitive qPCR.

MedixMDx qProbe Mix is a universal one-step probe mix for robust, sensitive, and fast qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase for real-time PCR amplification of single or multiplex DNA targets. MedixMDx qProbe Mix is compatible with several probes such as TaqMan® and Scorpions®.

MedixMDx qRT-Probe Mix is a universal one-step probe mix for robust, sensitive, and fast RT-qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and reverse transcriptase for efficient cDNA synthesis and real-time. The kit includes a ROX additive and an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.

MedixMDx HiPlex qRT-Probe Mix is an advanced formulated one-step qRT-PCR probe mix for highly sensitive, rapid, and robust detection of RNA target templates. MedixMDx HiPlex qRT-Probe Mix uses state of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and ultra-sensitive reverse transcriptase for efficient cDNA synthesis and real-time PCR amplification. MedixMDx HiPlex qRT-Probe Mix is formulated as a 4x mix, which enables extensive multiplexing and addition of larger volumes of RNA templates to reactions. It includes an efficient thermostable reverse transcriptase with RNase inhibitor to prevent degradation of RNA templates by RNases. MedixMDx HiPlex qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.

MedixMDx Fast Bst Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

MedixMDx Fast Bst RT Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst RT Mix also contains an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. MedixMDx Fast Bst RT Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Taq 2x PCR Master Mix is a ready-to-use reaction mix for sensitive, robust and fast PCR. The combination of aptamer-based fast-start Taq DNA polymerase, optimized reaction buffer and ulrapure dNTPs ensures consistent results, rapid set-up time and lower risk of pipetting errors. The Taq DNA polymerase has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. It provides a robust PCR performance for a wide range of PCR applications in all standard PCR cyclers.

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Taq 2x PCR Master Mix

PlexTaq® 5x qPCR Multiplex Master Mix is a ready-to-use reaction mix for sensitive and reliable probe-based multiplex qPCR in all standard real-time PCR cyclers. The combination of aptamer-based fast-start Taq DNA polymerase, optimized reaction buffer and ultrapure dNTPs, ensures consistent results, rapid set-up time and lower risk of pipetting errors. 5x concentration makes this mix optimal for multiplexing application, leaving more room for primers and probes.

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PlexTaq® 5x qPCR Multiplex Master Mix

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination is required. The combination of highly selective aptamer-based fast-start formulated HiDi® Taq DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. The variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.

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HiDi® Taq 2x PCR Master Mix

Volcano3G® RT-PCR Probe 2x Master Mix has all necessary components for sensitive and reliable RT-qPCRs. It includes an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. With the combination of optimized reaction buffer, ultrapure dNTPs and a blue stain for visualization, Volcano3G® RT-PCR Probe 2x Master Mix reduces the need for sample extraction and sample lysis. Volcano3G® RT-PCR Probe 2x Master Mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification.

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Volcano3G® RT-PCR Probe 2x Master Mix