分子诊断原料111

HiFi DNA Polymerase Hot-Start is an aptamer-based hot-start, high fidelity hyperthermophilic recombinant DNA polymerase from the archaeon Pyrococcus furiosus. HiFi DNA Polymerase Hot-Start exhibits 5' to 3' polymerase activity and 3' to 5' exonuclease activity. The optimized buffer chemistry facilitates high sensitivity, yield, specificity, robust and rapid polymerase processivity. The enzyme is ideal for long, complex, difficult DNA templates and is resistant to PCR inhibitors. HiFi DNA Polymerase Hot-Start has a lower error rate than standard Taq DNA polymerase (100x) and therefore, is suitable for PCR applications where higher accuracy is needed.

HighScript Reverse Transcriptase is a modified version of MMLV reverse transcriptase with noticeable thermostability and high enzymatic activity. This enzyme is offered as a blend with an RNase inhibitor to prevent RNA degradation. HighScript Reverse Transcriptase together with enhanced buffer chemistry enables fast, efficient and accurate synthesis of the cDNA molecule.

Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activitiy, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C.

Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Taq DNA polymerase Hot-Start is an aptamer-based fast-start formulated DNA polymerase supplied with a 10x reaction buffer that has been specially designed for optimal PCR performance and DNA polymerase activity. It's "Hot-Start" feature eliminates false amplification at temperatures below 50-55°C.

This DNA polymerase is suitable for a wide range of PCR applications and it's 5'-3'-exonuclease activity means it can be used for hydrolysis probe-based real-time PCRs.

For research use and further manufacturing. Designed and manufactured under ISO13485

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes.

For more information, please check :
RevTaq RT-PCR DNA polymerase, 50x concentrated glycerol stock

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).

Comparison studies with competitor products show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences.

By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.

Applications:

- Allele-specific PCR (asPCR), allele-specific amplification (ASA)

- HLA genotyping

- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)

- Mutation detection by PCR even in a high background of wild-type sequences

- Genotyping e.g., in CRISPR/Cas and TALEN approaches

This polymerase is also available as a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).

Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below.

For research use and further manufacturing. Designed and manufactured under ISO13485

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).

Comparison studies with competitor products show that the HiDi® Taq DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences.

By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.

Applications:

- Allele-specific PCR (asPCR), allele-specific amplification (ASA)

- HLA genotyping

- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)

- Mutation detection by PCR even in a high background of wild-type sequences

- Genotyping e.g., in CRISPR/Cas and TALEN approaches

This DNA polymerase is also available as a nuclease deficient variant, featuring higher robustness towards potential PCR inhibitors and compatibility with real-time dyes.

Several independently conducted studies show that HiDi® Taq DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below.

For research use and further manufacturing. Designed and manufactured under ISO13485